2024 Clontech y1h

Clontech y1h

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August undefined, 2024

WebNov 27, 2024 · Cloning of transcription factors (TFs) We employed the Yeast One-Hybrid assay (Y1H) (Clontech Y1H, Clontech, USA) to identify TFs that interact with the cloned … WebApr 30, 2024 · To further ascertain whether the specific binding of PavCYP78A9 was directly regulated by PavAGL15 in sweet cherry fruit, point-to-point Y1H experiments were performed to detect the interactions between PavAGL15 and the PavCYP78A9 promoter. The PavAGL15 protein directly bound to the promoter sequence of PavCYP78A9 in vitro …

Isolation of Plant Transcription Factors Using a Yeast One-Hybrid ...

http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf WebNov 1, 2004 · Gateway cloning technology compatible with Y1H was later developed, allowing high throughput for Y1H (Deplancke et al., 2004). Enhanced Y1H (eY1H) is a robotic mating platform that allows for fast ... thurles bookshop

pAbAi Sequence and Map - SnapGene

WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. WebI used the Matchmaker Gold yeast one-hybrid system from Clontech. ... The p53 fragment was from pGBKT7-p53 used for Y2H. I don’t know whether this fragment is suitable for Y1H. WebI used the Matchmaker Gold yeast one-hybrid system from Clontech. ... The p53 fragment was from pGBKT7-p53 used for Y2H. I don’t know whether this fragment is suitable for … thurleigh road medical centre

HbWRKY27, a group IIe WRKY transcription factor, positively …

WebThe yeast one-hybrid (Y1H) system is a powerful tool for the identification and isolation of cDNAs of transcription factors using promoter segments or regulatory elements as baits. Here we propose an adaptation of the Y1H system for identification and cloning of transcription factors using Matchmaker (Clontech) Y2H cDNA libraries. WebApr 1, 2016 · CLONTECH公司酵母单杂交试剂盒说明书.pdf.pdf. 2016-04-01上传. 暂无简介 thurleigh castleWebClontech Laboratories, Inc. www.clontech.com Protocol No. PT3529–1 A Takara Bio Company Version No. PR7Z2445 7 Matchmaker™ One-Hybrid Library Construction & … thurles community social services

"WebAug 6, 2024 · a Yeast one-hybrid (Y1H) analysis of DS1, OsARF11 and OsBRI1 promoter. The bait vector containing the OsBRI1 promoter fragment-fused lacZ reporter gene, ... (Clontech). Yeast transformation and screening procedures were performed according to the manufacturer’s instructions (Clontech). The yeast cells were transformed with the … " - Clontech y1h

Clontech y1h

Isolation of plant transcription factors using a yeast one-hybrid ...

WebA Y1H assay requires that a prey construct is introduced into a bait yeast strain that harbors the reporter construct(s). This is commonly achieved by transforming the expression construct into a haploid bait yeast strain. However, this can also be achieved using the ability of haploid yeast strains to mate and generate diploid yeast. Web菌落PCR产品，验证整合到Y1H酵母基因组的bait DNA序列 . 品牌：Clontech 技术资料：0 篇【商品总览】Clontech【630495】酵母双杂培养基套装（含 ...

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WebThe Y1H Gold strain is the compatible strain for pABAi vector provided by Clontech. Can we use Ym4271 (the strain for Y1H assay and has the same genotype of Y1H Gold … Web丁香通为您找到319条dna序列的联会信息，包括dna序列的联会报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购dna序列的联会,上丁香通。

WebOct 1, 2004 · We tested whether Gateway AttB recombination sites influence the readout of the Y1H system, for example, by binding an endogenous yeast TF. We compared our system to the commercially available Clontech Y1H system (BD Biosciences). As a positive control, we used the interaction between the TF p53 and its consensus DNA-binding site. WebOct 3, 2024 · To eliminate the effect of self-activation, the screening of Aureobasidin A (AbA) concentration was carried out. Then pAgFNS-ABAi vector and library plasmid DNA were co-transformed into yeast strain Y1H Gold (Clontech), and then cultured on selection medium (SD/Leu −) supplemented with or without AbA (150 ng/mL) for 3 days at 30 °C. We ...

WebClosed Date 2005. Founded Date 1974. Founders Roger Tsien. Operating Status Closed. Last Funding Type Private Equity. Company Type For Profit. Contact Email … WebC. elegans Transcription Factors have been cloned into Gateway-adapted vectors and can be used for yeast one-hybrid (Y1H) and two-hybrid (Y2H) assays. Utilizamos cookies propias y de terceros para mejorar nuestros servicios y mostrarle publicidad relacionada con sus preferencias mediante el análisis de sus hábitos de navegación.

WebApr 5, 2024 · Y1H assays. The full-length CDS of LkARF7 and LkARF19 were cloned into the prey vector pGADT7 individually, ... (Clontech, USA). The cDNA library was screened on SD/-Trp-Leu, SD/-Trp-Leu-His, and SD/-Trp-Leu-His-Ade/X-α-Gal medium to identify LkARF19-interacting proteins. The positive clones interacting with LkARF19 were …

WebJun 6, 2014 · Identification and cloning of tomato TCP genes. By mining the tomato Unigene and BAC sequence databases from the Sol Genomics Network with homology searches and subsequent sequence extension by RACE, we initially identified and cloned 24 different tomato genes encoding putative TCP transcription factors (Table 1; SlTCP1 … thurles dsuWebFeb 2, 2024 · The coding region of FtbZIP85 was cloned into pGBKT7 (Clontech, Mountain View, CA, USA) as the prey. The bait and prey constructs were co-transformed into the yeast strain AH109, ... (Y1H) assay to test whether FtbZIP85 could recognize the motifs in the FtDFR promoter. The results show that FtbZIP85 could bind to the bZIP/ABRE motif ... thurles cathedral live streamWebAdron Ung. So say that for an experiment which requires a [DNA] / [Protein] = 1.2 / 1 ratio, I have calculated that I need 96.2296 uM of duplex DNA. This double stranded DNA is formed by annealing ... thurles gaa twitterWeb2 days ago · Yeast one-hybrid (Y1H) assay. The promoter fragment of BoMY was obtained by PCR amplification. The amplified product was inserted into the pAbAi vector (Clontech, Palo Alto, CA, USA) to generate a proBoMY-pAbAi construct. The coding sequence of BoFAMA was cloned into the pGADT7 vector to generate pGADT7::BoFAMA … thurles credit union logoWebYNHH COVID19 Vaccination. 5%. The following questions should be answered on behalf of the individual being scheduled for vaccination. I am currently scheduling for: … thurles co. tipperaryWebIn the Y1H assay, potential target genes of a TF may be screened out by cis-element analysis. Similarly, the bind-ing site can also be identiﬁed with known TFs cDNA. Wang … thurles foot clinicWebOct 16, 2014 · Candidate genes identified by yeast one-hybrid (Y1H) screening ... (PT3530-5, Clontech), and the pre-prepared Y187 yeast competent cells. Transformants that grow in selective medium (S.D./- thurles eircode