Pip2-mcherry
WebbPlasmid pUBC_V5_MS2-mCherry from Dr. John Rinn's lab contains the insert MS2 and is published in Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 … Webb2 mars 2024 · The OsSCD2-GFP labeled punctate structures also partially colocalized with the PM marker PIP2-mCherry . In agreement with its localization in rice protoplasts, …
Pip2-mcherry
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WebbGmWAK1-GFP fusion protein was transiently expressed in Arabidopsis protoplasts; plasmid containing p2300-35S-H2B-mCherry (recombinant histone H2B) was … Webb6 okt. 2024 · The GFP signal observed in the PM protein enriched fraction provides another line of evidence to our confocal microscopy study observations which detected significant co-localization between the RabA2b GFP signal and the PIP2 mCherry fluorescence of . Coomassie brilliant blue was used to show equal loading of each sample .
WebbNational Center for Biotechnology Information Webb18 okt. 2011 · Single-particle tracking analysis revealed that PIP2;1 presented four diffusion modes with large dispersion of diffusion coefficients, suggesting that …
WebbmCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004). WebbPIP2;: mCherry), respectively. After 2 days, the uorescence signals were examined using laser confocal 3. uorescence microscopy (STELLARIS, Leica). Pi transport activity assay in yeast To generate vectors for yeast expression, the VPT1 and BnPHT5;1b coding regions were ampli ed from
WebbSimilarly, the signal of COLD1-GFP colocalized with that of PIP2-mCherry at ER with a reticular pattern and at the plasma membrane (Figures S4F, S4G, and 4D). The plasma …
Webb4 mars 2024 · pRhPTM::RhPTM-GFP and pRhPIP2;1::RhPIP2;1-mCherry were transiently co-expressed in tobacco leaves. Co-localizations of RhPTM-GFP and mCherry , GFP and … the fred hollowsWebbFor rubisco:mCherry, the protoplasts were isolated from the cotyledons of T 1 progeny because of the low levels of plastid contained in callus tissue. The mCherry alone was expressed in the nucleus and cytoplasm as a control. The red fluorescence of PIP2:mCherry targeting the PM was distributed as a single layer surrounding the … the fred hollows foundation bequestWebbmCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon … the adina hotelWebb28 mars 2024 · These constructs were individually transformed into Agrobacterium tumefaciens EHA105 cells, and the indicated combination of the constructs and the H2B-mCherry or PIP2-mCherry plasmids were transiently expressed in N. benthamiana leaves. The fluorescence signals were examined using a confocal laser scanning microscope … thead in html mdnWebbDownload scientific diagram Correlation/ colocalization analysis of secretory vesicles containing MMP9-GFP with lipid sensors tagged with a red-fluorescent protein: a PIP2 … thead in cssWebb25 maj 2011 · Phosphatidylinositol-(4,5)-bisphosphate (PIP2) is the main lipid binding partner of proteins involved in clathrin-mediated endocytosis (CME). ... affinity (unpublished data). We also did not detect enrichment of an mCherry fusion of the PIP 2-binding ANTH domain of CALM in CCPs (unpublished data), ... the fred hollows foundation hkWebb(A) 35S::PIP2;1-mCherry fusion protein. (B) 35S::EGFP fusion protein. (C) Bright-field. (D) Merged image of panels (A-C). (E) 35S::PIP2;1-mCherry fusion protein. (F) … the fred heutte center