WebbPick a single colony from a freshly streaked plate and inoculate a starter culture with 3 to 5 mL of media. Use the appropriate antibiotic and incubate at 37 °C* for approximately 8 hours while shaking at 250-300 rpm. Use the starter culture to inoculate an overnight culture. Dilute the starter culture 1:500 to 1:1000 in a large flask with the ... WebbContinue picking a single colony, restreaking and incubating plates until you have one where all the colonies growing have the same shape, size and color. Once you’re satisfied that you have a uniform plate of organisms, select one colony from the …
Plating Borellia burgdorferi for single colonies or after transformation
Webb26 nov. 2024 · Create Bacterial Glycerol Stock (BGS). Transfer BGS on a screwcap cryogenic tube and store in a -80°C freezer. Recover the bacteria by using a sterile loop and scrape off from frozen BGS. Streak the bacteria onto an agar plate and grow overnight in appropriate environment temperatures. WebbPlating Borrelia burgdorferi for single colonies or after transformation Basic protocol—for 16 plates. 1. Equilibrate 300 ml of 1.5x BSK + (stored frozen; see Recipes) and 200 ml … senior year high school supply list
Streaking for Single Colonies - YouTube
Webb28 okt. 2024 · The streaked plate is incubated at 37°C for 24 hours. Examine the colonies grown on the plate carefully. All colonies should have the same general appearance. If there is more than one colony … WebbMonoclonal liquid bacterial cultures should be started using single colonies. Watch this video to learn how to effectively streak and grow single colonies on plates. Show more … Webb767 views, 23 likes, 1 loves, 1 comments, 6 shares, Facebook Watch Videos from Jaguarpaw DeepforestSA: See No Evil 2024 S9E4 senior year love never fails